Updating and identifying three novel variants of the CARD14 gene in Chinese Han patients with psoriasis

Psoriasis-2 (PSORS2) is caused by the heterozygous mutation of the caspase recruitment domain 14 (CARD14) gene on chromosome 17q25. To evaluate the contribution of CARD14 variants in psoriasis of the Chinese Han population, we performed deep sequencing of the CARD14 gene in 372 Chinese Han patients with psoriasis. The exonic nucleotide variants were confirmed by Sanger sequencing in the affected individuals and 1114 controls. In 27 patients with psoriasis, we identified 15 variations, including three novel variants: c.381C[G (p.Cys127Trp), c.712A[G (p.Met238Val) and c.2260_2261delinsGG (p.Gln754Gly). These findings could enrich and update the Human Gene Mutation Database of CARD14 variants for psoriasis.

Three-dimensional finite element analysis of fibula implantation for China-Japan friendship hospital type osteonecrosis of femoral head / 中国组织工程研究

BACKGROUND: It is reported that the hip-conserving effect of osteonecrosis of femoral head is closely related to the retention of lateral column. The classification of China-Japan Friendship Hospital is based on the three-column structure, and the prediction accuracy of femoral head collapse is high. OBJECTIVE: To establish a three-dimensional finite element model for China-Japan Friendship Hospital classification of femoral head necrosis, and to analyze the mechanical changes of fibula implantation in each classification by finite element method, and to explore the significance of lateral column retention in hip preservation, so as to provide a basis for precise prediction of collapse of the classification. METHODS: Three groups of 11 kinds of three-dimensional finite element models of normal femoral head, China-Japan Friendship Hospital type femoral head necrosis (type M, type C, type L1, type L2, type L3) and fibula implantation were established. The finite element analysis was carried out by ANSYS software. The maximum stress, maximum displacement and load transfer mode of proximal femur were observed in each group. RESULTS AND CONCLUSION: (1) In the necrosis group, the strain was the largest, and the displacement was different due to the different types of necrosis. The displacement changes were as follows: Type M < type C < type L1 = type L2 < type L3. The displacement recovery of fibula implantation group was lower than that of the normal group, and the displacement recovery was different due to the different necrosis types. The displacement changes were as follows: Type M < type C < type L1 < type L2 < type L3. The reduction range of the displacement of the repaired necrotic femoral head gradually decreased from the lateral column to the medial column, which was lower than the maximum displacement of the normal femoral head. (2) The peak value of the stress nephogram of the loading area of the femoral head after necrosis was higher than that of the normal group. The peak value of necrotic type M was nearly normal. The peak value of necrotic type C was 74.5% higher than that of the normal group, and the peak value of necrotic type L was more than 100% higher than that of the normal group. The peak value of necrotic type M after operation was not only 14.2% lower than that before operation, but also was lower than that of the normal group. The peak value of necrotic type C after operation was 5.3% lower than that before operation, but higher than that of normal group. The peak value of necrosis type L after operation was lower than that before operation, but significantly higher than the normal level. (3) The load transfer in the normal femoral head was continuous. The conduction path was from the lateral column of the femoral head to the femoral moment. In the necrosis group, the internal load transfer of types M and C femoral head was continuous, and the conduction of type M was basically consistent with normal. The stress of type C conduction to femoral moment was reduced. The load transfer of L1, L2 and L3 type femoral head was interrupted. The stress changed in cliff type, and was unable to transmit to femoral moment, resulting in stress concentration in load area of the femoral head. A certain effective load transfer mode was reconstructed in the femoral head of the fibula implantation group, and the stress concentration at the femoral moment occurred in all types of conduction. Part of the load was transferred to the femoral moment through fibula, and the normal load transfer mode was partially restored. (4) China-Japan Friendship Hospital type fibula placement can prevent the collapse of the femoral head to a certain extent. The location and size of the necrosis area are very important. The closer the necrosis is to the lateral column, the easier it is to collapse and the more difficult it is to repair. The retention of the lateral column is an important factor for accurate prediction of the collapse of the femoral head.

Expressions of CD34, CD123 and CD38 in acute myelogenous leukemia and their clinical significances / 白血病·淋巴瘤

Objective To investigate the expressions of CD34, CD123 and CD38 in acute myelogenous leukemia (AML) and their clinical significances. Methods A total of 164 patients with AML in Xiangya Hospital of Central South University from February 2014 to July 2015 were enrolled. Cellular immunophenotyping was performed by flow cytometry. According to the expressions of CD34, CD38 and CD123, 164 patients were divided into positive group and negative group, and the clinical data and immature cells ratio of two groups were compared. Results In 164 patients with AML, 102 cases (62.2 ％) were CD34 positive, 126 cases (76.8％) were CD123 positive, and 144 cases (88.3％) were CD38 positive. There were no significant differences in age and sex between the positive and negative groups (P> 0.05). But there were significant differences in the proportion of immature cells, white blood cell count and hemoglobin between the two groups (all P< 0.05). The expression rates of CD34, CD38 and CD123 were correlated with minimal residual disease and complete remission rate (all P< 0.05). Conclusions CD34, CD123 and CD38 are effective markers for AML detection. The expressions of CD34, CD123 and CD38 can be used as the judgment marker of cell maturity, which is conducive to the determination of the condition and prognosis of AML patients.

Simulation analysis on optimial loading scheme of resetting tensile stress for clavicular fractures / 医用生物力学

Objective To investigate the optimal scheme about the external loading of resetting tensile stress for clavicular fractures, so as to provide some mechanical references for optimal design of external fixators for clavicular fractures. Methods The shoulder CT scanning data from a volunteer were used to establish the three-dimensional model of human left shoulder by Mimics software. In order to better simulate the actual human shoulder, the ligament tissues were added in the model and the constraints of muscles were considered, and the experimental model for simulation analysis on resetting tensile stress was established. By 3 simulation experiments, the impacts from directions of resetting tensile stress, positions of action points and value ranges were simulated and analyzed, respectively. The resetting tensile stresses obtained from clavicle of the shoulder model under various external loading cases were simulated by orthogonal test methods. Results When the (angle between the horizontal plane projection of shoulder force which was loaded by external fixator and the coronal axis ) was greater than 45°, clavicle could obtain the resetting tensile stress, which became greater with the angle increasing. When (angle between the sagittal plane projection of shoulder force which was loaded by external fixator and the sagittal axis) was 30°-45°, the resetting tensile stress of clavicle was the maximum. The farther the position on which the loads from external fixator was exerted from the horizontal position of clavicle, the larger the resetting tensile stress of clavicle was. Conclusions The results of orthogonal test show that the optimal resetting tensile stress is produced with parameter combination of θ=65°, η=50°, acting on the center of humerus head of the shoulder

Simulation analysis on optimial loading scheme of resetting tensile stress for clavicular fracture / 医用生物力学

Objective To investigate the optimal scheme about the external loading of resetting tensile stress for clavicular fracture,so as to provide some mechanical references for optimal design of external fixators for clavicular fracture.Methods The shoulder CT scanning data from a volunteer were used to establish the three-dimensional model of human left shoulder by Mimics software.In order to better simulate the actual human shoulder,the ligament tissues were added in the model and the constraints of muscles were considered,and the experimental model for simulation analysis on resetting tensile stress was established.By 3 simulation experiments,the impacts from directions of resetting tensile stress,positions of action points and value ranges were simulated and analyzed,respectively.The resetting tensile stresses obtained from clavicle of the shoulder model under various external loading cases were simulated by orthogonal test methods.Results When the θ (angle between the horizontal plane projection of shoulder force which was loaded by external fixator and the coronal axis) was greater than 45°,clavicle could obtain the resetting tensile stress,which became greater with the angle θ increasing.When η (angle between the sagittal plane projection of shoulder force which was loaded by external fixator and the sagittal axis) was 30°-45°,the resetting tensile stress of clavicle was the maximum.The farther the position on which the loads from external fixator was exerted from the horizontal position of clavicle,the larger the resetting tensile stress of clavicle was.Conclusions The results of orthogonal test show that the optimal resetting tensile stress is produced with parameter combination of θ =65°,η =50°,acting on the center of humerus head of the shoulder.

Simulation analysis on optimial loading scheme of resetting tensile stress for clavicular fracture / 医用生物力学

Objective To investigate the optimal scheme about the external loading of resetting tensile stress for clavicular fracture,so as to provide some mechanical references for optimal design of external fixators for clavicular fracture.Methods The shoulder CT scanning data from a volunteer were used to establish the three-dimensional model of human left shoulder by Mimics software.In order to better simulate the actual human shoulder,the ligament tissues were added in the model and the constraints of muscles were considered,and the experimental model for simulation analysis on resetting tensile stress was established.By 3 simulation experiments,the impacts from directions of resetting tensile stress,positions of action points and value ranges were simulated and analyzed,respectively.The resetting tensile stresses obtained from clavicle of the shoulder model under various external loading cases were simulated by orthogonal test methods.Results When the θ (angle between the horizontal plane projection of shoulder force which was loaded by external fixator and the coronal axis) was greater than 45°,clavicle could obtain the resetting tensile stress,which became greater with the angle θ increasing.When η (angle between the sagittal plane projection of shoulder force which was loaded by external fixator and the sagittal axis) was 30°-45°,the resetting tensile stress of clavicle was the maximum.The farther the position on which the loads from external fixator was exerted from the horizontal position of clavicle,the larger the resetting tensile stress of clavicle was.Conclusions The results of orthogonal test show that the optimal resetting tensile stress is produced with parameter combination of θ =65°,η =50°,acting on the center of humerus head of the shoulder.

Periodontal status of Pakistani orthodontic patients

The objective of this study was to evaluate and compare the periodontal status of orthodontic patients and non-orthodontic patients, aged 15–28 years, of both genders. The cross-sectional study included 100 orthodontic and 100 non-orthodontic patients evaluated using a Community Periodontal Index for Treatment Need (CPITN) probe on the index teeth. A questionnaire was distributed to the participants to assess and evaluate the use of oral hygiene aids. The data were analyzed using SPSS version 17, and various comparisons were performed using the chi-square test. The study revealed that there was a statistically significant association in CPITN scores between the orthodontic and non-orthodontic patients (p < 0.01). The study showed that patients undergoing orthodontic treatment have increased plaque accumulation and probing depth resulting in periodontal tissue destruction. Proper oral hygiene practices and interdental aids should be employed to control plaque.

Pterional keyhole approach in surgical treatment of ruptured anterior circulation intracranial aneurysm: a report of 313 cases / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To review the surgical modality with pterional keyhole approach in treatment of anterior circulation aneurysm.</p><p><b>METHODS</b>Three hundred and thirteen patients with ruptured anterior circulation intracranial aneurysm treated surgically with pterional keyhole approach between January 2009 and June 2014 in Department of Neurosurgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, were included in the analysis. Complete occlusion rate of aneurysms and incidence of major complications including delayed cerebral ischemia and chronic hydrocephalus were documented. Surgical outcomes at 6-month follow up were assessed by modified Rankin Scale.</p><p><b>RESULTS</b>Totally 348 aneurysms were treated with pterional keyhole approach, 326 aneurysms were completely clipped, 16 aneurysms were partly clipped, and 6 aneurysms were wrapped with gauze material. Among 313 patients, 15 patients (4.79%) suffered from delayed cerebral ischemia, and 10 patients (3.19%) suffered from hydrocephalus. At the 6-month follow up, the rate of good outcome was 66.77% (209/313).</p><p><b>CONCLUSIONS</b>The pterional keyhole approach can be used to clip most of anterior circulation aneurysms, and it seems to have advantages over the traditional approaches with lower incidence of complications and similar outcomes.</p>

Effects of Acupuncture Combined with Massage on Shoulder Subluxation after Hemiplegia / 中国康复理论与实践

@#ObjectiveTo observe the effects of acupuncture combined with massage on shoulder subluxation and motor function recovery of the upper extremities in patients with hemiplegia after stroke.Methods60 hemiplegic patients were randomly divided into two groups for treatment with acupuncture cooperates with massage or usual rehabilition, respectively. The recovery of the patient's shoulder subluxation and movement function of upper extremities were evaluated 2 months after treatment. ResultsThe shoulder subluxation and movement function of the upper extremities were improved after treatment with both therapies (P<0.01), and acupuncture cooperates with massage showed better effect (P<0.01). ConclusionAcupuncture cooperates with massage can facilitate the recovery of shoulder subluxation and motor function of the upper extremities after hemiplegia.